Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

A continuous authoritative sequence reviewing study into enzymology and similar parts of molecular biology. offers six papers through prime gurus.

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Mar are not O-methylated. In plants where O-methylation does occur, detailed substrate specificity studies may be necessary before implicating O-methyltransferase activity in the phytoalexin response; in callus cultures of Cunuuuliu ensifomis producing medicarpin (IX) in response to attack by Pithomyces chartarum, O-methyltransferase activity with caffeic acid or naringenin as substrate was not induced, although three- to fourfold increases in transferase activity were observed with 2',4,4'-trihydroxychalcone (XXVI), daidzein (XIX), or genistein (I) as substrates (210).

J. LAMB variety of potential pathogens. In addition to qualitative and quantitative variations in infective agent inoculum, localized conditions of incubation may also greatly influence the terpenoid accumulation in the host (249). The level at which the terpenoid phytoalexins accumulate in host tissue is likely to depend on the relative rates of their synthesis and degradation. Thus only a low level of rishitin (LXII) is observed in potato tubers 120-144 hr after inoculation with Phytophthoru infestuns or treatment with a cell-free extract from the fungus (250,25 1).

Jimsonweed (Datura stramonicum ) Source TABLE V (CONTINUED) cossypol (R,R' = OH) (LXXXVII) (LXXXVI) Momilactone B Cotton ( Gossypium SPPJ (LXXXV) Momilactone A (LXXXIII) Dehydroipomeamarone Rice (Oryza sativa) (LXXXII) lpomeamaronol Casbene (LXXXI) lpomeamarone Castor (Ricinus communis) Sweet potato (Ipomea batatas) (294,295) C. fimbriata wm ' / I / '/I/ R' (300-302) (300-302) (303) P. 291 ) C. w FKkAA Ceratocystis fimbnata h v) h v) 3 0 2 -m. 0 0 * . Q f Y Y 6 0f g d 2i P b 4 < 5 y^ X X 3 n B 5 0 a 56 57 PHYTOALEXINS: ENZYMOLOGY A N D MOLECULAR BIOLOGY Starch TCA cycle - 3 Glucose-6-phosphate +- 1 1 Acetyl CoA Pyruvate t -- - ~bulose-5-phosphate Fatty acids ~-HydroxymetliylglutarylCoA 1 Mevalonic acid 1 lsopen tenyl-pyrophosphate C 1 3,3’-diniethylallyl-pyrophosphate Geranyl-pyrophosphate -+ 1 - Monoterpenes I Farnesyl-pyrophosphate Sesquiterpenes - Geranylgeranyl-pyrophosphate Diterpenes Scheme 14.

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