A Field Guide to Animal Tracks by Olaus Johan Murie

By Olaus Johan Murie

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The pGp was then tested with Kaplan's specific rye grass 3'-nucleotide phosphatase (Shuster and Kaplan, 1953). >p as a check, being aware, however, of the fact that during such a procedure there would be an isomerization of the 2'- and 3'-positions. Figure 6 shows the time course of hydrolysis with the rye grass 3'-nucleotidase, and it can be seen that using for hydrolysis the product obtained with the enzymatic method (upper curve) thus retaining the original position, the nucleotidase completely hydrolyzes the radioactively marked phosphate, proving it to be attached in 3'-position.

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8 1 , 779-787. , Kohlbacher, M. , Shaw, S. , and Amos, H. (1972). Proc. Natl. Acad. Sei. A. 6 9 , 3 4 0 7 - 3 4 1 1 . Morse, M. , Lederberg, E. , and Lederberg, J. (1956). Genetics 41, 142-156. Nikaido, H. (1962). Proc. Natl. Acad. Sei. A. 48, 1337-1341. Nikaido, H. (1965). Bacterial cell wall-deep layers. In "The Specificity of Cell Surfaces" (B. D. Davis and L. ), pp. 3-30. Prentice-Hall, Englewood Cliffs, New Jersey. Ordal, G. , and Adler, J. (1974). / . Bacteriol. 117, 517-526. Osborn, M. , Rosen, M.

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